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APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria.

Dirkzwager RM, Kinghorn AB, Richards JS, Tanner JA

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  • Journal Chemical communications (Cambridge, England)

  • Published 09 Feb 2016

  • Volume 51

  • ISSUE 22

  • Pagination 4697-700

  • DOI 10.1039/c5cc00438a

Abstract

We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL(-1)) and could reliably diagnose malaria in clinical blood samples.